The anaplastic lymphoma kinase (ALK) gene is frequently involved in rearrangements that lead to gene fusions in lung cancer. Fusion partner of echinoderm microtubule-associated protein-like 4 (EML4) is frequently found in non-small-cell lung cancer (NSCLC). The recombinant EML4-ALK activates the receptor tyrosine kinase’s downstream signaling pathway, which includes PI3K/AKT, leading to carcinogenesis. It has been reported that the presence of the EML4-ALK fusion is correlated with the efficacy of 4LK-targeted therapy. Based on analysis of tumor messenger RNA, EML4-ALK fusions can be detected by real-time PCR method.
Pre-loaded and bulk formats are available.
Compatible with tissue samples.
Top sensitivity of 25 copies/rxn.
Two steps procedure, results in 150 mins.
External control included.
EML4-ALK Fusion Gene Detection Kit is a real-time PCR assay for qualitative detection of 21 EML4-ALK fusions in total RNA extracted from formalin-fixed paraffin-embedded (FFPE) tumor tissue in NSCLC patients.
The kit is for research use only and intended to be used by trained professionals in a laboratory environment.